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| Genome Wide Expression Profile of Asiasarum sieboldi in LPS-stimulated BV-2 Microglial Cells. |
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글쓴이 : 최고관리자
날짜 : 08-10-02 10:54
조회 : 1919
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 생리2008-05.pdf (139.9K), Down : 15, 2008-10-02 10:54:24 | |
Sung-Hwa Sohn1,2, Eunjung Ko1,Yangseok Kim1, Minkyu Shin1,2,Moochang Hong1 & Hyunsu Bae1,2
1)Department of Physiology, College of Oriental Medicine,KyungHee University, Seoul 130-701, Korea
2)BK21 Oriental Medical Science Center, KyungHee University,Seoul 130-701, Korea
Abstract
Recent studies suggest that activated microglial cells play an essential role in the inflammatory responses and neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease. This study was conducted to evaluate the protective mechanisms of Asiasarum sieboldi (AS) on LPS-induced activation of BV-2 microglial cells. The effects of AS on gene xpression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100 mm dish (1×107/ mL) for 24 h and then pretreated with 1 μg/mL AS or left untreated for 30 min. Next, 1 μg/mL LPS was added to the samples and the cells were reincubated at 37C for 30 min and 1 hr. The gene expression profiles of the BV-2 microglial cells varied depending on the AS. The microarray analysis revealed that MAPK signaling pathway-related genes were downregulated in AS-treated BV-2 microglial cells. AS can affect the neuroinflammatory-related pathway such as MAPK signaling pathway in activated BV-2 microglial cells.
*Correspondence and requests for materials should be addressed to H. S. Bae (hbae@khu.ac.kr)
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